Crop Improvement Research Articles

Response of grain amaranth (Amaranthus hypochondriacus) to irrigation schedules under sprinkler irrigation

A field experiment was conducted during winter (rabi) seasons of 2019–20 and 2020–21 at the Centre for Crop Improvement, Sardarkrushinagar Dantiwada Agricultural University, Banaskantha, Gujarat to study the response of grain amaranth (Amaranthus hypochondriacs L.) to irrigation schedules under sprinkler irrigation system. The experiment was conducted in a randomised block design (RBD) comprised of 6 irrigation treatments, viz. I0 , Surface irrigation; I1 , Control (only pre-sowing irrigation); I2 , Sprinkler irrigation at 0.2 IW: CPE; I3 , Sprinkler irrigation at 0.4 IW: CPE; I4 ,Sprinkler irrigation at 0.6 IW: CPE; and I5 ,Sprinkler irrigation at 0.8 IW:CPE with 4 replications. The results revealed that application of irrigation at IW: CPE ratio of 0.8 had significant effect on plant height, inflorescence length (83.4 cm), grain yield (1,625 kg/ha) and straw yield (7,497 kg/ha) compared with control treatment (surface irrigation). Treatment 0.8 IW: CPE resulted in the 28.15% higher grain yield than the control. The highest water-use efficiency (WUE) (12.8 kg/ha-mm) was achieved by the treatment I1 (only pre-sowing irrigation). Consumptive use (mm) was found increased with increase in irrigation scheduling from I0 to I5 . The highest consumptive use of 179.3 mm was recorded under the treatment of 0.8 IW: CPE ratio. Irrigation at 0.8 IW: CPE ratio fetched highest net returns of `54,701/ha, with benefit: cost (B:C) ratio 3.06 during both the years in pooled analysis.

Uncovering DNA methylation landscapes to decipher evolutionary footprints of phenotypic diversity in chickpea.

Genetic diversity and environmental factors are long believed to be the dominant contributor to phenotypic diversity in crop plants. However, it has been recently established that, besides genetic variation, epigenetic variation, especially variation in DNA methylation, plays a significant role in determining phenotypic diversity in crop plants. Therefore, assessing DNA methylation diversity in crop plants becomes vital, especially in the case of crops like chickpea, which has a narrow genetic base. Thus, in the present study, we employed whole-genome bisulfite sequencing to assess DNA methylation diversity in wild and cultivated (desi and kabuli) chickpea. This revealed extensive DNA methylation diversity in both wild and cultivated chickpea. Interestingly, the methylation diversity was found to be significantly higher than genetic diversity, suggesting its potential role in providing vital phenotypic diversity for the evolution and domestication of the Cicer gene pool. The phylogeny based on DNA methylation variation also indicates a potential complementary role of DNA methylation variation in addition to DNA sequence variation in shaping chickpea evolution. Besides, the study also identified diverse epi-alleles of many previously known genes of agronomic importance. The Cicer MethVarMap database developed in this study enables researchers to readily visualize methylation variation within the genes and genomic regions of their interest (http://223.31.159.7/cicer/public/). Therefore, epigenetic variation like DNA methylation variation can potentially explain the paradox of high phenotypic diversity despite the narrow genetic base in chickpea and can potentially be employed for crop improvement.

Insight into the genomic diversity: Genome wide association study unveils the SNP diversity in horsgram germplsam

Genome wide association study (GWAS) was performed to identify loci associated with three agronomic traits namely seeds per pod, pods per plant and number of seeds per plant of horsegram (Macrotyloma uniflorum). Total 87 accesions of horsegram from different geographical regions of India and one wild relative from Australia were utilised for the genome wide association studies. The genotyping was carried out using genotype-by-sequencing technology and after high quality filtering, minor allele frequency of 0.05, a total of 55,656 quality SNPs were utilised for determining Marker-Trait Association. The LD decay varied across the chromosomes form 8 kb to 300 kb. Further, the population structure analysis has clustered the accession into three subpopulations. All the 88 accessions of horsegram were evaluated at Palampur (32.1109° N, 76.5363° E) and Bajaura (31.8465°N, 77.1605°E) locations of North-Western Himalayan region, India from. In the current study, 143 MTAs associations were observed for seeds per pod distributed on all 8 chromosomes, 2 for pods per plant at chromosomes 2 and 3 and 1 for number of seeds per plant at chromosome 7. However, total 11 stable QTLs (9 for number of seeds per plant and 2 for pods per plant) were also identified. These QTLs can serve as a potential source for marker assisted selection. The genes identified within and in proximity to the stable QTLs will serve as a bridge between genetic information and its practical application for crop improvement.

Sustainability concern in Indian agriculture: needs science-led innovation and structural reforms

Agriculture in India is facing several challenges which together are manifested into the sustainability issues. The broad contours of the agricultural production system in the country have been defined by the need to achieve food security which calls for close attention to rice–wheat (Triticum aestivum L.) cropping system of the Indo Gangetic Plains (IGP) whose sustainability is under threat. Degradation of natural resources, severe biotic and abi otic stresses specially drought, floods, pest infestations with accompanying impacts on biodiversity and agricultural productivity are the major constraints to agricultural development. Further, climate change has gained significant global attention over the past decade due to concerns of deleterious long-term impacts on agriculture, water sup ply, human welfare, regional and political stability. All the related issues need to be addressed on priority, with par ticular emphasis on soil-carbon through a holistic approach. As the strategy brings focus on income of farmers, the agricultural technology needs to move from “Production oriented-Green Revolution” to “Farmer’s Income oriented Revolution” and environmentally sustainable farming. Therefore, for long-term sustainability in agriculture, 4 pil lars/ components (good agricultural practices, climate-change mitigation and adaptation, diversification of high value crops and biodiversity management) needs to be addressed properly, more importantly good agricultural practices (GAP). The GAPs are based on the principles of risk prevention, risk analysis, sustainable agriculture, and integrated crop management (ICM), which are of utmost importance in present time. The GAPs for agricultural sustainability are reduced tillage, conservation agriculture, resource-conservation technologies (RCTs), erosion control measures, diversified cropping system, micro-irrigation, balanced fertilization, manuring, watershed man agement, organic farming, and integrated farming systems etc. Now that the vision is to impart income security to the farmers of the country, diversification of the system across all the sub-sectors of agriculture assumes impor tance which is, indeed a de-risking mechanism capable of negotiating both endogenous and exogenous risks as sociated with the system. The effective and efficient management of agro-biodiversity is also essential through management of genebanks, science-led innovations; livelihood, food and nutrition security though crop diversifica tion, use of lesser-known crops and wild relatives in crop improvement; dealing appropriately with quarantine, bio safety and bio-security.

Induced Mutagenesis using Gamma Rays: Biological Features and Applications in Crop Improvement

Induced mutagenesis has emerged as an essential field of plant breeding to address global food security challenges, offering tools to enhance crop productivity, resistance, and nutritional value. Among the mutation induction tools, the physical mutagen such as gamma rays holds promise for efficient mutation induction. Gamma rays, a type of high-energy electromagnetic radiation, possess unique characteristics that enable them to penetrate plant tissues and induce genetic mutations. The biological effects are influenced by various factors, including the source, type of radiation, dose rate, absorbed dose, and the sensitivity of the tissues and organs. Gamma rays have been successfully applied to various plant species, producing novel superior mutants for cultivation. This paper explores the different aspects of gamma irradiation, including the radiation facilities, the biological effects of gamma rays on plant species, and the potential applications to generate genetic diversity and unlock desirable trait improvement in crop plants. The paper also showcases successful examples of high-yielding mutants developed through gamma-ray mutagenesis and their impact on agriculture. The potential approach of plant mutagenesis offers to address challenges for plant improvement for sustainable and resilient food production.

Targeted C•G-to-T•A base editing with TALE-cytosine deaminases in plants

BackgroundTALE-derived DddA-based cytosine base editors (TALE-DdCBEs) can perform efficient base editing of mitochondria and chloroplast genomes. They use transcription activator-like effector (TALE) arrays as programmable DNA-binding domains and a split version of the double-strand DNA cytidine deaminase (DddA) to catalyze C•G-to-T•A editing. This technology has not been optimized for use in plant cells.ResultsTo systematically investigate TALE-DdCBE architectures and editing rules, we established a β-glucuronidase reporter for transient assays in Nicotiana benthamiana. We show that TALE-DdCBEs function with distinct spacer lengths between the DNA-binding sites of their two TALE parts. Compared to canonical DddA, TALE-DdCBEs containing evolved DddA variants (DddA6 or DddA11) showed a significant improvement in editing efficiency in Nicotiana benthamiana and rice. Moreover, TALE-DdCBEs containing DddA11 have broader sequence compatibility for non-TC target editing. We have successfully regenerated rice with C•G-to-T•A conversions in their chloroplast genome, as well as N. benthamiana with C•G-to-T•A editing in the nuclear genome using TALE-DdCBE. We also found that the spontaneous assembly of split DddA halves can cause undesired editing by TALE-DdCBEs in plants.ConclusionsAltogether, our results refined the targeting scope of TALE-DdCBEs and successfully applied them to target the chloroplast and nuclear genomes. Our study expands the base editing toolbox in plants and further defines parameters to optimize TALE-DdCBEs for high-fidelity crop improvement.

Self-(in)compatibility in Tunisian apple accessions [Malus domestica. Borkh]: S-genotypes identification and pollen tube growth analysis.

Self-incompatibility studies have revealed a potential use of Tunisian apple resources for crop improvement and modern breeding programs and a likely correlation between the pollen tube growth and flowering period. Apples [Malus domestica. Borkh] exhibit an S-RNase-based gametophytic self-incompatibility (GSI) system. Four primer combinations were used to S-genotype eighteen Tunisian local apple accessions and twelve introduced accessions that served as references. Within the Tunisian local accessions, S2, S3, S7, and S28 S-alleles were the most frequent and were assigned to 14 S-genotypes; among them, S7S28, S3S7, S2S5, and S2S3 were the most abundant. PCA plot showed that population structuring was affected by the S-alleles frequencies and revealed a modern origin of the Tunisian varieties rather than being ancient ones. Nonetheless, the results obtained with 17 SSR markers showed a separate grouping of local Tunisian accessions that calls into question the hypothesis discussed. Pollination experiments showed that the pollen started to germinate within 24h of pollination but 48h after pollination in the "El Fessi" accession. The first pollen tubes arrived in the styles within 36h of pollination in two early flowering accessions known as "Arbi" and "Bokri", and after 72h of pollination in late flowering "El Fessi" and 48h after pollination in remaining accessions. The first pollen tube arrests were observed in accessions "Arbi" and "Bokri" within 84h of pollination, within 108h of pollination in "El Fessi" and within 108h of pollination in remaining accessions. In the apple accession called "Boutabgaya," the pollen tubes reached the base of the style within 120h of pollination without being aborted. Nevertheless, the self-compatible nature of "Boutabgaya" needs more studies to be confirmed. However, our results revealed the malfunction of the female component of the GSI in this accession. To conclude, this work paved the path for further studies to enhance the insight (i) into the relation between the flowering period and the pollen tube growth, (ii) self-compatible nature of "Boutabgaya", and (iii) the origin of the Tunisian apple.

Physiological and proteomic characterization revealed the response mechanisms underlying aluminium tolerance in lentil (Lens culinaris Medikus).

Aluminium (Al) toxicity causes major plant distress, affecting root growth, nutrient uptake and, ultimately, agricultural productivity. Lentil, which is a cheap source of vegetarian protein, is recognized to be sensitive to Al toxicity. Therefore, it is important to dissect the physiological and molecular mechanisms of Al tolerance in lentil. To understand the physiological system and proteome composition underlying Al tolerance, two genotypes [L-4602 (Al-tolerant) and BM-4 (Al-sensitive)] were studied at the seedling stage. L-4602 maintained a significantly higher root tolerance index and malate secretion with reduced Al accumulation than BM-4. Also, label-free proteomic analysis using ultra-performance liquid chromatography-tandem mass spectrometer exhibited significant regulation of Al-responsive proteins associated with antioxidants, signal transduction, calcium homeostasis, and regulation of glycolysis in L-4602 as compared to BM-4. Functional annotation suggested that transporter proteins (transmembrane protein, adenosine triphosphate-binding cassette transport-related protein and multi drug resistance protein), antioxidants associated proteins (nicotinamide adenine dinucleotide dependent oxidoreductase, oxidoreductase molybdopterin binding protein & peroxidases), kinases (calmodulin-domain kinase & protein kinase), and carbohydrate metabolism associated proteins (dihydrolipoamide acetyltransferase) were found to be abundant in tolerant genotype providing protection against Al toxicity. Overall, the root proteome uncovered in this study at seedling stage, along with the physiological parameters measured, allow a greater understanding of Al tolerance mechanism in lentil, thereby assisting in future crop improvement programmes.

Enhancing microspore embryogenesis in diploid potato (Solanum tuberosum L. ssp. tuberosum) germplasm

Doubled haploidy (DH) technology has been utilized in cultivated tetraploid potato (Solanum tuberosum L. ssp. tuberosum) to accelerate crop improvement; however very little work has been done with the diploid species. Experiments were undertaken to improve microspore embryogenic response in the diploid germplasm. Several factors influencing embryogenic responses were evaluated. An increase in calcium nitrate, a reduction in the plant growth regulators 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA), as well as an incubation temperature of 28 °C resulted in an increase in callus production and, in some cases, embryo-like structures. Validation of the modified protocol was conducted with both diploid and tetraploid potato germplasm with responses from both diploid and tetraploid. Monoploid and di-haploid plants were also regenerated from these microspore-derived calli.

Applicability of SCoT markers in unraveling genetic variation and population structure among sugar beet (Beta vulgaris L.) germplasm.

Sugar beet (Beta vulgaris L.) holds significant importance as a crop globally cultivated for sugar production. The genetic diversity present in sugar beet accessions plays a crucial role in crop improvement programs. During the present study, we collected 96 sugar beet accessions from different regions and extracted DNA from their leaves. Genomic DNA was amplified using SCoT primers, and the resulting fragments were separated by gel electrophoresis. The data were analyzed using various genetic diversity indices, and constructed a population STRUCTURE, applied the unweighted pair-group method with arithmetic mean (UPGMA), and conducted Principle Coordinate Analysis (PCoA). The results revealed a high level of genetic diversity among the sugar beet accessions, with 265 bands produced by the 10 SCoT primers used. The percentage of polymorphic bands was 97.60%, indicating substantial genetic variation. The study uncovered significant genetic variation, leading to higher values for overall gene diversity (0.21), genetic distance (0.517), number of effective alleles (1.36), Shannon's information index (0.33), and polymorphism information contents (0.239). The analysis of molecular variance suggested a considerable amount of genetic variation, with 89% existing within the population. Using STRUCTURE and UPGMA analysis, the sugar beet germplasm was divided into two major populations. Structure analysis partitioned the germplasm based on the origin and domestication history of sugar beet, resulting in neighboring countries clustering together. The utilization of SCoT markers unveiled a noteworthy degree of genetic variation within the sugar beet germplasm in this study. These findings can be used in future breeding programs with the objective of enhancing both sugar beet yield and quality.

Advancements in genetic techniques and functional genomics for enhancing crop traits and agricultural sustainability.

Genetic variability is essential for the development of new crop varieties with economically beneficial traits. The traits can be inherited from wild relatives or induced through mutagenesis. Novel genetic elements can then be identified and new gene functions can be predicted. In this study, forward and reverse genetics approaches were described, in addition to their applications in modern crop improvement programs and functional genomics. By using heritable phenotypes and linked genetic markers, forward genetics searches for genes by using traditional genetic mapping and allele frequency estimation. Despite recent advances in sequencing technology, omics and computation, genetic redundancy remains a major challenge in forward genetics. By analyzing close-related genes, we will be able to dissect their functional redundancy and predict possible traits and gene activity patterns. In addition to these predictions, sophisticated reverse gene editing tools can be used to verify them, including TILLING, targeted insertional mutagenesis, gene silencing, gene targeting and genome editing. By using gene knock-down, knock-up and knock-out strategies, these tools are able to detect genetic changes in cells. In addition, epigenome analysis and editing enable the development of novel traits in existing crop cultivars without affecting their genetic makeup by increasing epiallelic variants. Our understanding of gene functions and molecular dynamics of various biological phenomena has been revised by all of these findings. The study also identifies novel genetic targets in crop species to improve yields and stress tolerances through conventional and non-conventional methods. In this article, genetic techniques and functional genomics are specifically discussed and assessed for their potential in crop improvement.

Genetic diversity analysis and molecular characterization of tropical rose (Rosa spp.) varieties

Characterization of rose varieties becomes essential for understanding, utilizing, and preserving the diversity among them, for their genetic improvement, cultivation, and conservation. With this purpose, 19 tropical rose varieties were evaluated via quantitative morphological and molecular markers. A significant degree of variation was observed across nearly all descriptors, suggesting a notable level of genetic diversity among the released rose varieties. Traits like flower diameter, flower yield/plot, and shelf life, considered to be of vital importance given the aesthetic value of rose, exhibited remarkable differences that would further aid in the selection of desirable varieties. The maximum flower diameter, flower yield/plot, and shelf life were recorded in Yellow Page, Lambada, and Delhi Princess, respectively. The varieties were evaluated by correlation matrix, hierarchical agglomerative clustering method-based dendrogram, network plot on phenotypic correlation, colour intensity graph including Red-Green-Blue (RGB) scattered plot. In the correlation matrix, it was found that more number of primary shoot contributes to higher flower yield, while increase in leaf area degraded the shelf life of the flower. Realizing the importance of molecular characterization, 10 selected polymorphic inter simple sequence repeats (ISSR) and start codon targeted (SCoT) polymorphism primers were tested that amplified total 187 polymorphic loci with 94.4 % polymorphism. Cent percent polymorphism were found with SCoT-2, SCoT-18, SCoT-19, HB-12 and ISSCR3 primers. A principal component analysis based on molecular diversity highlighted that First Red, Local Selection 2, and Himroz exhibited distinct characteristics compared to the other varieties. The 19 varieties were grouped into 8 clusters viaunweighted pair group method with arithmetic mean-based dendrogram at 0.4 similarity co-efficient. The analysis revealed a significant genetic distance between Himroz, First Red, and Grand Gala, suggesting their potential to be utilized in hybridization programs for crop improvement. The distinguishable genetic background and high degree of variation revealed by molecular markers, specifically ISSR and SCoT, offer valuable insights that can contribute to the strategic improvement of roses. These findings provide a foundation for expediting rose breeding programs and comprehensive crop improvement strategies.

Advancements of Breeding and Genomics in Wheat (Triticum aestivum L.): Enhancing Yield and Nutritional Value for Sustainable Agriculture

Advancements in wheat breeding and genomics presently explores the genomic interventions driving focusing on quantitative trait loci (QTL) mapping, marker-assisted selection (MAS) and genomic selection (GS). QTL mapping emerges as a pivotal method for pinpointing markers linked with desirable traits, facilitating MAS. Furthermore, genomic selection (GS) holds immense potential for crop improvement. It also delves into the current landscape of MAS and explores various prospects of GS for wheat biofortification. Looking ahead, accelerated mapping studies combined with MAS and GS schemes are poised to further enhance wheat breeding efficiency. Dense molecular maps and a large set of ESTs (Expressed Sequence Tags) have enabled genome-wide identification of gene-rich and gene-poor regions, as well as QTL, including eQTL (Expression quantitative trait loci). Additionally, markers associated with major economic traits have facilitated MAS programs in some countries and enabled map-based cloning of several genes/QTL. Resources for functional genomics, such as TILLING and RNA interference (RNAi), alongside emerging approaches like epigenetics and association mapping, are further enriching wheat genomics research. In this review, we initially present cutting-edge genome-editing technologies in crop plants, with a specific focus on wheat, addressing both functional genomics and genetic enhancement. We subsequently delineate the utilization of additional technologies, including GWAS, high-throughput genotyping and phenotyping, speed breeding, and synthetic biology, within the context of wheat breeding. We assert that integrating genome editing with other molecular breeding strategies will significantly expedite the genetic enhancement of wheat, thus contributing to sustainable global production.

Understanding of G × E interactions of yield attributes in soybean MAGIC population and characterization for charcoal rot resistance

AbstractMulti‐parent advanced generation inter‐cross (MAGIC) populations have been developed and utilized in crop improvement programs in several economically important crops. In the current study, soybean (Glycine max L.) MAGIC population was evaluated at four locations to identify stable and high‐yielding MAGIC RILs (recombinant inbred lines) and to understand the genotype and environmental interaction for grain yield and attributing traits. For different traits under study, pooled analysis of variance indicated the significant genotype × environmental interactions at p < 0.01. RILs such as G236, G455, G159, G493, and G488 found ideal for grain yield across the four environments. Through multi‐trait genotype‐ideotype distance index ideotype‐MAGIC RILs have been identified for each location. Significant positive correlation of grain yield with pods per plant, 100‐seed weight, plant height, number of nodes per plant, and branches per plant has been identified. Under field conditions, screening of F2:3 generation of MAGIC population revealed that only 59 progenies exhibited adult plant resistance for charcoal rot disease (caused by Macrophomina phaseolina). Although under controlled conditions parental genotypes EC 572136 and EC 572109 found to be partially resistant with least mean necrosis length, MAGIC RILs (F2:8) showed variable reaction. Thus, the study established diverse genetic resources useful for both mapping and varietal development program.

[Hemicellulose modification and cell wall genetic improvement in plants].

Hemicellulose, as a primary component of plant cell walls, constitutes approximately one third of cell wall dry matter and ranks as the second abundant renewable biomass resource in the nature after cellulose. Hemicellulose is tightly cross-linked with cellulose, lignin and other components in the plant cell wall, leading to lignocellulose recalcitrance. However, precise genetic modifications of plant cell walls can significantly improve the saccharification efficiency of lignocellulose while ensuring normal plant growth and development. We comprehensively review the research progress in the structural distribution of hemicellulose in plant cell walls, the cross-linking between hemicellulose and other components of the cell wall, and the impact of hemicellulose modification on the saccharification efficiency of the cell wall, proving a reference for the genetic improvement of energy crops.

Role of Genome Sequences of Major and Minor Millets in Strengthening Food and Nutritional Security for Future Generations

Millets are small-seeded cereals belonging to the family Poaceae. They are considered to be climate-resilient and future nutritional food cereals for humans. Millets are resistant to biotic and abiotic stressors compared to other major cereals and thrive in low-quality soils with little maintenance and less rainfall. The importance of millets is still not well known to many people due to the lack of popularity and cultivation in semi-arid tropics of Asia and Africa. The United Nations has declared 2023 as the International Year of Millets (IYM 2023) to promote millet cultivation and popularize their health benefits globally. A few years ago, the application of molecular biology was in its infancy in millets due to the unavailability of genome sequences. Genome sequences are available for most of the millets on NCBI and Phytozome databases. In this review, we discuss the details of genome sequences for millets, candidate genes identified from the native genome of millets. The current status of quantitative trait loci and genome-wide association studies in millets are also discussed. The utilization of millet genome sequences in functional genomics research and translating the information for crop improvement will help millet and non-millet cereals survive harsh environments in the future. Such efforts will help strengthen food security and reduce malnutrition worldwide in 2050.

Targeted genome-modification tools and their advanced applications in crop breeding.

Crop improvement by genome editing involves the targeted alteration of genes to improve plant traits, such as stress tolerance, disease resistance or nutritional content. Techniques for the targeted modification of genomes have evolved from generating random mutations to precise base substitutions, followed by insertions, substitutions and deletions of small DNA fragments, and are finally starting to achieve precision manipulation of large DNA segments. Recent developments in base editing, prime editing and other CRISPR-associated systems have laid a solid technological foundation to enable plant basic research and precise molecular breeding. In this Review, we systematically outline the technological principles underlying precise and targeted genome-modification methods. We also review methods for the delivery of genome-editing reagents in plants and outline emerging crop-breeding strategies based on targeted genome modification. Finally, we consider potential future developments in precise genome-editing technologies, delivery methods and crop-breeding approaches, as well as regulatory policies for genome-editing products.

Genetic Engineering in Indian Mustard (Brassica juncea L.): Current Progress and Future Directions for Enhanced Crop Improvement

Genetic tools have revolutionized mustard improvement strategies, offering innovative avenues to enhance its agronomic traits and quality parameters. CRISPR technology, exemplified by research at the National Institute of Plant Genome Research in India, has enabled precise manipulation of glucosinolate levels in mustard plants, improving mustard oil quality. The Barnase-barstar gene system has facilitated the development of mustard hybrids, increasing yield and resilience. Additionally, Microsatellites have emerged as indispensable tools for understanding genetic relationships within mustard populations. These genetic tools hold promise for addressing agronomic challenges and meeting market demands in mustard cultivation. However, their deployment raises ethical, regulatory, and socio-economic considerations that require careful consideration. Responsible stewardship and transparent deployment of these technologies are essential to realize their full potential in enhancing mustard crops and ensuring a sustainable future for food production. In conclusion, genetic engineering offers exciting avenues for mustard improvement, with CRISPR, the Barnase-barstar gene system, and Microsatellites playing pivotal roles in enhancing crop quality, yield potential, and resilience. As mustard continues to play a crucial role in global agriculture and food security, the responsible utilization of these genetic tools holds promise for meeting the evolving needs of farmers and consumers worldwide. Furthermore, the paper briefly discusses the application of these genetic tools in enhancing Dhara Mustard, a popular variety in Indian agriculture, emphasizing its potential impact on addressing agricultural challenges and meeting consumer demands.

A novel four‐gene‐based inheritance model explains gradations of nakedness in cotton (Gossypium hirsutum L.)

AbstractIn crop improvement, delineating inheritance pattern of a character guides a plant breeder to choose appropriate breeding procedure, which enables ease of improving phenotypic expression of the traits. To deduce the inheritance pattern of naked seed or fuzzless character, four parental lines involving two purely naked seed types (DHN‐1 and DHN‐10), one partial naked seed type (SP46CC) and one fully fuzzy type (OSB415) were used to develop three different types of crosses, namely, naked × naked seed type cross (N × N), partial naked × naked seed type cross (PN × N) and fuzzy × naked seed type cross (F × N). We observed that naked trait segregates in the ratio of 11 naked: 5 partial naked in N × N cross, 22 naked: 42 partial naked in PN × N cross and 22 naked: 42 partial naked: 192 fuzzy in F × N cross. Our study reports a novel four‐gene model to understand inheritance nature of naked seed character where the presence of single basic dominant fuzzy gene negates the effect of genes controlling nakedness resulting fuzzy phenotype irrespective of the number of partial fuzzyness/nakedness imparting alleles. This indicates that nakedness is recessive to fuzzyness. Based on our results, we infer observing gradations of nakedness on seeds depends on the presence of number of partial fuzzyness imparting alleles, which led to a conclusion that the trait exhibited dose‐dependent expression with one basic gene for fuzziness.

In silico analysis and designing gRNA constructs for the precise modification of the OsTMS5 gene in rice (Oryza sativa L.): a comprehensive study and construct development for crop improvement

In silico analysis and designing gRNA constructs for the precise modification of the OsTMS5 gene in rice (Oryza sativa L.): a comprehensive study and construct development for crop improvement